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DE111 Probiotic helps balance gut microbiota. Bacillus subtilis probiotic DE111 delivers immune and digestive health benefits in supplement, food and beverage, and pet nutrition applications.

DE111 is a Bacillus subtilis probiotic that supports

How might DE111 benefit your supplement formulation?

The benefits of B. subtilis are well-supported. They’re supported by our expert team at Deerland and validated IRB-approved, double-blind, randomized, and placebo controlled clinicals.

In fact, we’ve invested in more than 30 studies to confirm the safety and efficacy of Bacillus subtilis DE111. While this species of microorganism has been known for more than 100 years and DE111 has GRAS status, at Deerland, we like to take validation a step further.

And by “a step,” we mean a full genome sequencing, which confirmed the strain contained no plasmids, no antibiotic resistant genes, and no deleterious genes. The genome sequence of DE111 has been uploaded to GenBank, the National Institutes of Health genetic sequence database.

It’s safe. And it works.

Because we’re probiotic people, WE LIKE TO DIG INTO WHY DE111 WORKS SO WELL.

Bacillus subtilis is a spore-forming (good) bacterium. Spore formers are characterized by their ability to form endospores to protect themselves in varying conditions.

This means DE111 is more stable than many other probiotic strains. It doesn’t require refrigeration, withstands high temperatures often associated with food and beverage manufacturing processes, and maintains viability through a wide pH range.

In stability testing, DE111 experienced virtually no loss of colony forming units (CFU) over 24 months when stored at room temperature (25 °C).

DE111 remains stable over 24 months at 25 °C and 40% relative humidity.

DE111 viability is maintained under temperatures up to 100 °C for up to 5 minutes.

DE111 viability is maintained under a wide pH range for a period of three weeks.

Not only does DE111 withstand processing, it survives the acidic environment of the gut to deliver meaningful benefits.

  • Produces many enzymes, several of which help break down food, including proteins the body has trouble digesting
  • Has beneficial effects on the immune system

How might DE111 benefit your formulation?


30+ studies confirm safety and efficacy
Full genome sequencing
FDA-Notified GRAS
Organic compliant
Star-K Kosher certified
Non-GMO Project Verified
Health Canada approved natural product
Non-novel food status in Canada, Australia, and New Zealand
NASC preferred supplier